Charlie Carter’s injury-time goal saw the Spitfires secure a late 1-1 draw against Dagenham and Redbridge.
The midfielder scrambled home the ball from a late corner to cancel out Paul McCallum’s strike earlier in the half to ensure the spoils were shared at the Silverlake.
Lee Bradbury made two changes from the side that beat Wealdstone 1-0 on Saturday.
George Langston returned to the starting line-up in place of the injured Aaron Martin and Ousseynou Cissé replaced John Oyenuga. There was also a place in the squad for summer signing Nigel Atangana for the first time.
Those changes meant Eastleigh lined up with a back four, rather than the back five they had deployed from the start of the last two fixtures.
Eastleigh started the stronger of the two sides. JJ McKiernan showed early signs of danger when he released Brennan Camp down the right whose teasing ball evaded the blue shirts in the box.
The Spitfires’ first real chance on goal fell to Ryan Hill, who had picked up a position on the edge of the box, with enough around him he looked to curl the ball into the corner.
Instead, his effort stung the palms of Elliot Justham who remained standing directly in the centre of the goal.
It was then the visitor’s turn to go close from range, Joe McDonnell got down to his right quickly to palm away Mohammed Sagaf’s long range drive.
After a slow start to the second half the visitors found the breakthrough thanks to Paul McCallum.
The ball bounced about in the box until it found Junior Morias in space on the left, the Jamaican cut the ball back for McCallum who slotted home from close range.
Daryl McMahon’s side continued to probe for a second and went extremely close to doing so when Mauro Vilhete struck the woodwork with an effort from range.
That was the trigger for Bradbury to make a double change, Cissé and Jake Hesketh making way for Oyenuga and Oscar Rutherford.
With seconds left on the clock McDonnell came rushing up from his goal for a late corner.
The ball flicked off the head of the goalkeeper and eventually fell at the feet of Carter who remained composed amongst the chase to slide the ball over the line.
There was just enough time left for another Spitfires corner, Panter’s expert delivery finding the head of McKiernan whose header looped onto the top of the net.
Eastleigh Line-up: McDonnell, Camp, Langston, Panter, Harper, Cissé (Rutherford, 78′), Carter, Hesketh (Oyenuga, 78′), McKiernan, Hill, Abrahams
Subs: Flitney, Atangana, Willson
Dagenham and Redbridge Line-up: Justham, Harem, Johnson, Sagaf, McCallum, Weston, Robinson, Tavares, Vilhete, Longe-King, Morais (Walker, 87)
Subs: Strizovic, Rance, Onariase, Zouma
Match report by George Rees-Julian